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NUTRIENT BIOMARKER

Vitamin D

25-Hydroxyvitamin D (25-OH-D)

The storage form of vitamin D - core nutrient status marker, deficient in ~40% of adults.

STANDARD RANGE
30–100 ng/mL (sufficient by lab standards)
OPTIMAL (OPTIMIZATION)
50–80 ng/mL is a common optimization target
RANGE VISUALIZATION

How Vitamin D ranges relate

The standard lab range vs the optimization-focused target. Illustrative trajectory shows what a 12-week improvement pattern looks like — not real user data.

30100STANDARD LAB RANGEOPTIMALWK 0WK 12ILLUSTRATIVE TRAJECTORY (NOT REAL DATA)
Standard lab rangeOptimization-focused targetIllustrative trajectory
TRACKED IN PROTOCOLS FOR
Build MuscleRecover FasterSleep BetterCognitive PerformanceLongevitySkin & AestheticsGut HealthImmunityMood & StressWomen's Longevity

What Vitamin D Measures

25-hydroxyvitamin D (25-OH-D) is the circulating storage form of vitamin D and the standard biomarker of vitamin D status. Values <20 ng/mL indicate deficiency; 20–29 insufficiency; 30+ is the standard sufficiency threshold. Optimization-focused practitioners typically target 50–80 ng/mL based on observational data linking higher levels with reduced CVD, cancer, and all-cause mortality.

Vitamin D is fat-soluble and status is driven primarily by sunlight exposure, dietary intake (fatty fish, fortified dairy), and supplementation. Deficiency is shockingly common - 30–50% of adults in temperate latitudes, higher in people with darker skin or limited sun exposure.

What Affects This Biomarker

Vitamin D status is influenced by: sun exposure (UVB), latitude + season, skin pigmentation (darker skin requires more sun), age (older skin makes less D per exposure), body fat (fat-soluble - high adiposity sequesters D), kidney function, and supplementation. Most people need 2000–5000 IU/day oral D3 + K2 to hit 50+ ng/mL.

In the Context of Peptide Protocols

Track at baseline on every protocol - D status affects testosterone, immune function, and bone health independent of any peptide. Re-test 8–12 weeks after starting supplementation to ensure dose is adequate. Pair with calcium, phosphorus, and PTH if values are extreme.

Related Reading

Conditions That Track Vitamin D

RECOVERY
Chronic Fatigue / Low Energy
Persistent unexplained fatigue - always requires a clinician workup. Tracked patterns give context to those conversations.
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Informational only - not medical advice. Reference ranges vary by lab and individual context. Work with a licensed provider to interpret your specific results.