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TB-500 Reconstitution & Dosage Calculator

TB-500 is a synthetic version of Thymosin Beta-4 promoting cell migration, angiogenesis, and wound healing. Forms the Wolverine Stack with BPC-157.

Half-life: HoursFrequency: 2x weekly loading, 1x weekly maintenanceRoute: Subcutaneous or intramuscular

TB-500 Reconstitution Calculator

Pre-filled with standard TB-500 values. Adjust as needed.

Typical range: 2–2.5mg loading, 1–2mg maintenance
RECONSTITUTION PROCESS
BAC H2O5mg1. DRAW 2mL BAC2. INJECT INTO VIAL3. SWIRL TO DISSOLVE4. DRAW 1.00mL DOSE
5mg + 2mL → 2500 mcg/mL
Concentration2500 mcg/mL
Draw volume1.000 mL
Draw to (100-unit syringe)100.0 units
Total doses per vial2 doses
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TB-500 Dosing Guide

Loading: 2–2.5mg 2x weekly for 4–6 weeks. Maintenance: 1–2mg once weekly. Wolverine Stack = TB-500 + BPC-157 for synergistic tissue repair.

Blood markers to track

hs-CRPCBC with differentialSerum copper

Note: Synergistic with BPC-157. TB-500 handles cell migration while BPC-157 drives angiogenesis.

Frequently Asked Questions

What is the standard TB-500 dose?

Loading: 2–2.5mg 2x weekly for 4–6 weeks. Maintenance: 1–2mg once weekly. Wolverine Stack = TB-500 + BPC-157 for synergistic tissue repair.

How do I reconstitute TB-500?

Add 2mL of bacteriostatic water to a 5mg vial of TB-500 to get a concentration of 2500 mcg/mL. This gives 2500mcg per 100 units on a 100-unit insulin syringe.

What blood markers should I track while running TB-500?

Common biomarkers tracked alongside TB-500 protocols include hs-CRP, CBC with differential, Serum copper. Establishing a baseline panel before starting and re-drawing labs at consistent intervals lets you measure changes against your own reference point, generic lab ranges are less informative than your personal trend over time. MyProtocolStack tracks each of these biomarkers in the context of your active TB-500 protocol.

Last reviewed: June 2026. For education and tracking only, not medical advice. Confirm any dose with a licensed healthcare provider.

WHAT TO DO NEXT

You have the dose. Now track whether it’s doing anything.

Log every TB-500 injection alongside your hs-CRPand CBC with differential readings. Compare draws over time in your free dashboard, so the next change you make is based on your numbers, not a guess.

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TB-500 protocol guides

BPC-157 vs TB-500: Which Do You Need?

Read guide →

BPC-157 + TB-500 Stack Guide

Read guide →

Wolverine Stack vs Glow Stack

Read guide →
FREE COMPANION GUIDE
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NOT MEDICAL ADVICE. FOR INFORMATIONAL USE ONLY. CONSULT A LICENSED HEALTHCARE PROVIDER. · All Calculators · Blog · Home