Peptide Reconstitution Calculator: The Complete Guide
Step-by-step guide to reconstituting lyophilized peptides with BAC water. Includes syringe unit calculations and storage protocols.
Why Reconstitution Matters Most research peptides arrive lyophilized -- freeze-dried into a powder. Before injection, they must be dissolved in bacteriostatic water (BAC water). Get this calculation wrong and you will under-dose or overdose every injection. The MyProtocolStack reconstitution calculator handles this automatically, but understanding the math makes you a better, safer user.
The Core Formula
Concentration = Peptide Amount (mcg) divided by BAC Water Added (mL)
Dose volume (mL) = Target Dose (mcg) divided by Concentration (mcg/mL)
Insulin syringe units = Dose volume (mL) multiplied by 100
Common Reconstitution Examples
5mg BPC-157 vial plus 2 mL BAC water = 2,500 mcg/mL concentration. At 250 mcg/dose: draw 10 units on an insulin syringe. At 500 mcg/dose: draw 20 units.
2mg Tesamorelin vial plus 1 mL BAC water = 2,000 mcg/mL concentration. At 1mg (1,000 mcg) dose: draw 50 units. At 2mg dose: draw 100 units.
2mg Ipamorelin vial plus 2 mL BAC water = 1,000 mcg/mL concentration. At 200 mcg dose: draw 20 units. At 300 mcg dose: draw 30 units.
BAC Water vs Sterile Water
Always use bacteriostatic water (BAC water) -- not sterile water, not saline. BAC water contains 0.9% benzyl alcohol which prevents microbial growth and extends reconstituted peptide shelf life to 30-60 days under refrigeration. Sterile water is single-use only.
Storage After Reconstitution
Refrigerate at 2-8 degrees C (standard fridge shelf). Never freeze after reconstitution -- ice crystal formation degrades the peptide. Protect from light. Label with date. Discard if cloudy, discolored, or particulate.
Using the MyProtocolStack Calculator
Visit myprotocolstack.com/calculators. Enter your vial size (mg), BAC water volume (mL), and target dose (mcg). The calculator instantly shows concentration, draw volume in mL, and exact insulin syringe units -- no math required.
The information in this article is for educational purposes only. Always consult a licensed healthcare provider before starting any peptide protocol.
Written by Ryan -- Founder, MyProtocolStack. Last Updated: April 2026.
Enter your blood work in MyProtocolStack, run StackAI analysis, and get personalized insights based on your actual numbers — not generic charts.
Start Free →