TB-500
Systemic cell-migration and tissue-repair peptide, most commonly stacked with BPC-157 for the "Wolverine" recovery protocol.
43 residues· First described 1981
Active fragment of Thymosin Beta-4 (43-residue full protein)
Overview
TB-500 is a synthetic analog of Thymosin Beta-4, a naturally occurring actin-sequestering protein that plays a central role in cell migration, angiogenesis, and wound healing. While BPC-157 drives angiogenesis and gut-mucosal repair, TB-500 handles cellular migration across tissues - which is why they're often stacked ("Wolverine Stack") for synergistic effect on connective-tissue injuries that neither compound alone resolves as quickly.
TB-500 is particularly useful in injuries where the damage is systemic or spans multiple structures - post-surgical rehab, chronic tendinopathy, multi-joint overuse. Its long systemic effect profile makes it injectable less frequently than BPC-157 (typically 2× weekly during loading, dropping to weekly after).
Because the primary signal is functional (pain, ROM, return-to-sport) rather than a single moving biomarker, tracking TB-500 requires protocol discipline - dose dates logged, pain/ROM tracked in parallel, and hs-CRP as the systemic inflammation read-through.
Mechanism of Action
TB-500 sequesters actin monomers in the cytoplasm, which liberates cells to migrate to sites of injury. It upregulates VEGF, stimulates endothelial cell proliferation and differentiation, and accelerates conversion of myoblasts to muscle fiber. Effects are broadly distributed via the circulatory system rather than localized to injection site, unlike BPC-157's stronger local signal.
Community Usage Patterns
Loading protocol: 2–2.5 mg SC or IM, 2× per week for 4–6 weeks. Maintenance: 1–2 mg once weekly. Most protocols continue for 8–16 weeks depending on injury. Stacks extremely well with BPC-157 500 mcg daily for soft-tissue injury. Injection can be abdomen, glute, or near injury site - site matters less than with BPC-157 because the effect is systemic.
Education only - not medical advice. Any protocol change should involve your licensed provider.
Biomarkers to Track
When running TB-500, these are the biomarkers most commonly tracked to assess response and safety:
Reconstitution Calculator
Free calculator for TB-500 reconstitution math - vial size, BAC water volume, and exact syringe units.
Open TB-500 calculator →Side Effects & Monitoring
What TB-500 side effects are commonly reported, when they appear, the red flags worth a provider call, and the biomarkers that catch issues early.
TB-500 side effects & what to track →Results & What to Track
What TB-500 results actually look like in the data - the markers to measure, when they tend to move, and how to tell a real result from placebo.
TB-500 results & how to measure them →Related Peptides
Stacks That Include TB-500
TB-500 Head-to-Head Comparisons
Conditions TB-500 is Commonly Researched For
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Start tracking →This page is informational and does not constitute medical advice. MyProtocolStack is a tracking and education platform. Work with a licensed provider before starting, changing, or stopping any protocol.