Longevity: Anti-Aging Peptides, Supplement Stack & Biological Age Markers

The short version

Longevity isn't a single compound. It's metabolic health, low inflammation, preserved muscle mass, sleep quality, and a handful of compounds with research-grade evidence for slowing biological aging. The protocol on this page is built from peer-reviewed research on NAD+ precursors, sirtuin activators, mitochondrial peptides, and the biomarkers that actually correlate with all-cause mortality.

Why this protocol works

The longevity foundation is unglamorous: NMN (NAD+ precursor with strong published RCT data), resveratrol (sirtuin activation, requires fat for absorption), vitamin D3 + K2 (cardiovascular + bone + immune), omega-3 EPA/DHA (target Omega-3 Index 8–12% per Framingham data), and magnesium glycinate. Berberine cycled 8 weeks on / 4 weeks off provides AMPK activation similar to metformin without the prescription.

Epithalon is the most-researched longevity peptide — published Russian data going back to the 1980s shows telomere elongation in human studies. MOTS-c is the mitochondrial peptide that's emerged in the last decade with strong metabolic-health data. GHK-Cu has published cellular-rejuvenation data and is in the longevity protocol of multiple respected clinicians.

The biomarkers that predict longevity outcomes — independent of chronological age — are ApoB (cardiovascular), HbA1c + fasting insulin (metabolic), hs-CRP (inflammation), homocysteine (cognitive + cardiovascular), albumin (nutritional/inflammatory), and biological-age markers like the PhenoAge composite. These are the panel any longevity-focused user should track quarterly.

Peptides commonly used for longevity

Supplement stack pairing

Biomarkers to track for longevity

The protocol

1. 1
   Baseline comprehensive longevity panel: full lipids inc. ApoB + Lp(a), HbA1c, fasting insulin, hs-CRP, homocysteine, vitamin D, B12, albumin, ferritin.
2. 2
   Longevity Foundation supplement stack: NMN 500mg morning, resveratrol 500mg with fat, D3 5000 IU + K2 100mcg morning, fish oil 2g EPA+DHA with meals, magnesium glycinate 400mg bedtime, berberine 500mg 2x with meals (8 on / 4 off).
3. 3
   Epithalon 5–10mg daily SubQ for 10–20 days, twice yearly (the standard published protocol).
4. 4
   Resistance train 3–4x/week. Sarcopenia is the largest preventable longevity risk after age 50.
5. 5
   Sleep 7+ hours. The sleep-longevity correlation is one of the strongest in epidemiology.
6. 6
   Re-test the full panel quarterly. Trends over time matter more than any single value.

Common pitfalls

• ×Stacking 30 supplements without measuring anything. The "more is better" approach is how people end up with high homocysteine because they're pounding niacin without B12.
• ×Ignoring ApoB. The single best cardiovascular risk marker; standard lipid panels skip it.
• ×Skipping the boring stuff (sleep, training, stress) for the exotic stuff (peptides, NAD+ IVs). The boring stuff has the strongest data.
• ×Treating biological age tests as gospel. They're useful for trend-tracking, not as precise individual estimates.

Other goals